Hydrodynamic injection with pneumatic valving for microchip electrophoresis with total analyte utilization
نویسندگان
چکیده
منابع مشابه
Advancements in capillary-assembled microchip (CAs-CHIP) development for multiple analyte sensing and microchip electrophoresis.
This review describes advancements toward the development of a capillary-assembled microchip (CAs-CHIP) for simultaneous multiple analyte sensing and microchip capillary electrophoresis. Development of such an advanced system relies on several factors such as improving the fluid handling technique, creating new biosensing mechanisms, and integrating different functional capillaries into a singl...
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A hybrid microchip/capillary electrophoresis (CE) system was developed to allow unbiased and lossless sample loading and high-throughput repeated injections. This new hybrid CE system consists of a poly(dimethylsiloxane) (PDMS) microchip sample injector featuring a pneumatic microvalve that separates a sample introduction channel from a short sample loading channel, and a fused-silica capillary...
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Here we show that a closed pneumatic microvalve on a PDMS chip can serve as a semipermeable membrane under an applied potential, enabling current to pass through while blocking the passage of charged analytes. Enrichment of both anionic and cationic species has been demonstrated, and concentration factors of ~70 have been achieved in just 8 s. Once analytes are concentrated, the valve is briefl...
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A novel microchip capillary electrophoresis system with electrochemical detection, using the replaceable microelectrode, is first reported. This kind of electrode can be fabricated in general laboratories and can be replaced quickly with electrodes of different materials according to the requirements of experiments. The end-column electrochemical detection on microchip CE was achieved by fixing...
متن کاملStudy of injection bias in a simple hydrodynamic injection in microchip CE.
The electrokinetically pinched method is the most commonly used mode for sample injection in microchip capillary electrophoresis (microCE) due to its simplicity and well-defined sample volume. However, the limited injection volume and the electrophoretic bias of the pinched injection may limit its universal usage to specific applications. Several hydrodynamic injection methods in microCE have b...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2011
ISSN: 0173-0835
DOI: 10.1002/elps.201000522